Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  :10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity. 
Tren is an extremely androgenic hormone, and as a result well known for promoting androgenic side effects. Hair loss and acne in men who are predisposed are both possible. Body hair growth is also possible. If you are not genetically predisposed no amount of Tren on earth with cause these issues. The androgenic side effects of Tren are truly based on genetic response. When it comes to hair loss, if you are predisposed to male pattern baldness, meaning at some point you’re going to lose your hair regardless of steroid use , Tren is one of the fastest ways to ensure you lose your hair a little sooner.
Virilization is a serious androgenic side effect of Tren. It is very difficult for a woman to use Tren without some level of virilization and makes it a steroid that’s almost never recommended for female use.
Important Note: The 5-alpha reductase enzyme does not metabolize the Trenbolone hormone. This means 5-alpha reductase inhibitors like Finasteride will not affect the androgenicity of the hormone.
As alluded to above, one very important thing to acknowledge when using AAS (whether taking one hormone, stacking or cycling) is the risk of harmful side effects. Within a steroid cycle, the users will often stack other non-anabolic hormones into their program to maximize specific cycle objectives for example: the addition of drugs like Clenbuterol and/or Cytomel /T3 augment cutting/definition cycles; others called aromatase inhibitors (estrogen reducing drugs) like Letrozole . Letro and Anastrozole Arimidex are often included to inhibit the conversion of excess testosterone to negatively cycle impacting estrogen and; incorporating post-cycle therapy (PCT) drugs such as the synthetic estrogens Tamoxifen . Nolvadex , or Clomiphene Citrate . Clomid (which act as anti-estrogens in the male body), can be used alone, together, or in conjunction with those like Mesterolone . Proviron and Human Chorionic Gonadotropin ( HCG ) during PCT to bridge the gap between the end of a steroid cycle (synthetic testosterone usage) and the restoration of the bodys natural testosterone production. These drugs too must be researched, and controlled in similar fashion to AAS. Thus, steroid cycles can be as simple or complex as the users individualized goals, cycle histories and levels of understanding. Below are three samples of AAS stacked cycles of varying complexity along with a beginning PCT sample, and an explanation of goal intention & rationale for the selected compounds, dosages & durations. These illustrations and commentaries will provide a better understanding of what stacking and cycling are along with the many nuances they require.